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產(chǎn)品名稱(chēng): 膠原蛋白酶 來(lái)源于溶組織梭菌
產(chǎn)品型號(hào): V900891
產(chǎn)品展商: sigma
產(chǎn)品文檔: 無(wú)相關(guān)文檔
簡(jiǎn)單介紹
產(chǎn)品品牌：sigma Vetec 產(chǎn)品名稱(chēng)：膠原蛋白酶 來(lái)源于溶組織梭菌 別名: 梭菌肽酶 A 英文名稱(chēng)：Collagenase from Clostridium histolyticum Type I, for general use, Vetec? reagent grade, powder, ≥125 CDU/mg solid 產(chǎn)品編號(hào) V900891 | CAS號(hào) 9001-12-1 規(guī)格：100mg 1g 膠原蛋白酶 來(lái)源于溶組織梭菌
膠原蛋白酶 來(lái)源于溶組織梭菌
的詳細(xì)介紹
膠原蛋白酶 來(lái)源于溶組織梭菌 膠原蛋白酶 來(lái)源于溶組織梭菌 膠原蛋白酶 來(lái)源于溶組織梭菌
Biochem/physiol Actions
Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.
Quality
也具有梭菌蛋白酶、非特異***蛋白酶和胰蛋白酶活性。
Unit Definition
One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.
Application
Collagenase from Clostridium histolyticum may be used for the isolation of cells from many types of animal tissue. VETEC Collagenase Type I has the original balance of collagenase, caseinase, clostripain, and tryptic activities, and is suggested for epithelial, liver, lung and adrenal primary cell isolations.
性質(zhì)
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grade
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Vetec? reagent grade
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form
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powder
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storage temp.
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?20°C
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膠原蛋白酶 來(lái)源于溶組織梭菌
膠原蛋白酶 來(lái)源于溶組織梭菌