如果您對該產品感興趣的話,可以
產品名稱: 膠原蛋白酶 來源于溶組織梭菌
產品型號: V900892
產品展商: sigma
產品文檔: 無相關文檔
簡單介紹
產品名稱：膠原蛋白酶 來源于溶組織梭菌 英文名稱：Collagenase from Clostridium histolyticum Type II, Vetec? reagent grade, powder, ≥125 CDU/mg solid 產品編號 V900892 | CAS號 9001-12-1 | VETEC 規格：100mg 1g 膠原蛋白酶 來源于溶組織梭菌
膠原蛋白酶 來源于溶組織梭菌
的詳細介紹
膠原蛋白酶 來源于溶組織梭菌 膠原蛋白酶 來源于溶組織梭菌 膠原蛋白酶 來源于溶組織梭菌
Biochem/physiol Actions
Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.
Quality
也具有梭菌蛋白酶、非特異***蛋白酶和胰蛋白酶活性。
Unit Definition
One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.
Application
Collagenase from Clostridium histolyticum may be used for the isolation of cells from many types of animal tissue. VETEC Collagenase Type II has higher clostripain activity, and is suggested for bone, heart, liver, thyroid and salivary primary cell isolations.
性質
|
grade
|
Vetec? reagent grade
|
|
form
|
powder
|
|
storage temp.
|
?20°C
|
膠原蛋白酶 來源于溶組織梭菌 膠原蛋白酶 來源于溶組織梭菌