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超濾離心管

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產品名稱: 超濾離心管
產品型號: UFC901096
產品展商: 密理博
產品價格: 0.00 元
會員價格: 0.00 元
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簡單介紹

Millipore超濾管，15ml 10K


超濾離心管  的詳細介紹
產品名稱 :Millipore超濾管
 
品牌 :密理博
 
貨號 :UFC901096
 
規格 :15ml 10K
 
 
  • Amicon Ultra-4* and -15* Centrifugal Filter Units are designed for in vitro diagnostic use and intended for concentrating serum, urine, cerebrospinal fluid and other body fluids prior to analysis. 
  • The premier devices for concentrating 1 – 4mL or <15 mL 
  • High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs 
  • High retentate recovery of >90% 
  • Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes) 
  • Heat-sealed membrane minimizes downstream extractables 
  • 100% integrity tested for reliable performance 
  • Convenient sample monitoring with translucent housing and volume gradations 
  • Direct pipettor sample access eliminates processing step to recover concentrate 
  • High concentration factors of 80–100X

 

The Amicon Ultra-4 and Amicon Ultra-15 with Ultracel-10 membrane are CE marked.

Applications

 

  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms 
  • Purification of macromolecular components found in tissue culture extracts or cell lysates 
  • Concentration of dilute or pre-purified proteins from column eluents 
  • Desalting and buffer exchange

 

Performance data - Amicon Ultra-4 Centrifugal Filter Unit

Typical Spin Times to Filtrate Volume

 

Spin conditions: 4000 xg swinging bucket rotor at 25 ° C, 4 mL sample. 
3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).

Typical Protein Recovery

Membrane
NMWL, kDa 		Protein Solute Protein Retentate
Volume, mL 		Protein Retentate
Recovery, %
10 Cytochrome c, 12.4 kDa
(0.25 mg/mL) 		0.20 95
4 mL starting volume in a fixed angle rotor at 7,500 x g at 25 °C. Spin times: 3K (30 min); 10 and 30K (10 min); 50K (5 min); 100K (15 min).

Performance data - Amicon Ultra-15 Centrifugal Filter Unit

Typical Spin Times to Filtrate Volume

 

Spin conditions: 4000 xg swinging bucket rotor at 25° C, 15 mL sample. 
3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).

Typical Protein Recovery

Membrane
NMWL, kDa 		Protein Solute Protein Retentate
Volume, mL 		Protein Retentate
Recovery, %
10 Cytochrome c, 12.4 kDa
(0.25 mg/mL) 		0.24 93.03
15 mL starting volume in swinging bucket rotor at 4000 x g at 25 °C. Spin times: 3K (40 min); 10K and 100K (20 min); 30K (10 min); 50K (15 min).
 
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